Insulin internalization in human erythrocytes

Insulin radioreceptor assay for human erythrocytes.

Human erythrocytes have specific insulin receptors. A radloreceptor assay for the determination of Insulin binding to these receptors is presented. After two passages over a Boyum-type adient, erythrocytes from freshly collected heparinized blood were isolated and 3.5 X iO erythrocytes per milliliterwere incubated for 2.5 h in a modified pH 8.0 4-(2-hydroxyethyl)1-piperazine ethane sulfonate bu...

Insulin binding and insulin-dependent phosphorylation of the insulin receptor solubilized from human erythrocytes.

Insulin receptors were extracted from human erythrocytes contained in 100 ml of blood with the nonionic detergent Triton X-100 with almost 100% yield. The solubilized receptor had binding characteristics similar to those of the intact cell. Using 125I-monoiodoinsulin as tracer and a computer-assisted statistical curve-fitting program, a cooperative model gave values of 1.7 X 10(9) M-1 for the K...

The influence of tracers on insulin binding to human erythrocytes.

We studied insulin binding to human erythrocytes using two different 125I-insulin-tracers. Erythrocytes of 8 normal subjects were examined using [mono-125II-(Tyr A 14)insulin as tracer. Three of these erythrocyte preparations were examined simultaneously using [125I]insulin, which was randomly iodinated by the chloramine-T-method. Data were analysed by a computerized non-linear least-squares pr...

Insulin binding to erythrocytes in diabetes mellitus.

Insulin binding to erythrocytes was studied in diabetic patients. Insulin binding was lower in untreated diabetics and diabetic patients treated with diet or insulin than in normal subjects. Binding variation was mainly due to decreased binding site concentration in untreated and insulin-treated patients, and to lowered insulin binding site affinity in diet-treated patients. Several patients tr...

Adenosine Metabolism in Human Erythrocytes